PCB 3023L - Cell Biology Laboratory

College of Natural Sciences

Credit(s): 1
Contact Hours: 45
Effective Term Fall 2024 (640)

Requisites

Pre- or Co-requisite BSC 3017 with a minimum grade of C and
Pre- or Co-requisite PCB 3023 with a minimum grade of C and
(Prerequisite CHM 2210 with a minimum grade of C and
Prerequisite CHM 2210L with a minimum grade of C) and
((Prerequisite BSC 2010 with a minimum grade of C and
Prerequisite BSC 2010L with a minimum grade of C) or
Prerequisite BSC 2010CH with a minimum grade of C)

Course Description

This laboratory course will consist of experiments that teach and apply fundamental techniques used in cell biology such as isolation and study of cellular organelles, sub-cellular fractionation, protein isolation, detection and activity. (Note: Credit is not given for (PCB 3023 and PCB 3023L) and (PCB 3023C).

Learning Outcomes and Objectives

  1. The student will illustrate advanced techniques of microscopy that provide an understanding of various cell types and methods of identification by:
    1. identifying various cell types.
    2. examining sub-cellular structures using microscopic morphology.
  2. The student will accurately perform standard cell biology protocols that focus on cellular protein isolation and analysis of protein activity by:
    1. isolating sub-cellular fractions of homogenized cells using differential centrifugation.
    2. separating proteins using various chromatography techniques.
    3. examining protein composition of isolated sub-cellular fractions using gel electrophoresis, protein analysis and quantitative enzyme assays.
  3. The student will accurately analyze and interpret scientific data to gain confidence and skill in problem solving techniques by:
    1. performing experiments, collecting and organizing data, assessing the validity of the data, and drawing appropriate conclusions based on statistical evaluation of the results.
    2. correlating data to scientific literature and evaluating its relevance.
    3. presenting results through written and oral communication.

Criteria Performance Standard

Upon successful completion of the course the student will, with a minimum of 70% accuracy, demonstrate mastery of each of the above stated objectives through laboratory measures developed by individual course instructors.

History of Changes

C&I Approval: 05/16/2014, BOT Approval: 10/21/2014, Effective Term: Spring 2015 (495). C&I Approval: , BOT Approval: , Effective Term: Spring 2019 (555). C&I Approval: 09/05/2019, BOT Approval: 09/24/2019, Effective Term: Spring 2020 (570).
C&I Approval: 02/17/2024, BOT Approval: 03/19/2024, Effective Term: Fall 2024 (640)

Related Programs

  1. Biology (BIOLOGY-BS) (670) (Active)
  2. Biology (BIOLOGY-BS) (640) (Draft)
  3. Laboratory Specialist (LAB-ATC) (670) (Active)